recombinant human light tnfsf14 protein  (R&D Systems)

Journal: PLOS One

Article Title: Genetic ablation of interleukin-17A augments fibrosis in a mouse model of cholestatic liver injury

doi: 10.1371/journal.pone.0342251

Figure Lengend Snippet: A. The abundance of total IHL isolated from WT and Il-17a -/- mice is quantified (N = 4-6 per group). B. Total number of IHL isolated from WT and Il-17a -/- mice reared on control or DDC diet is normalized to liver weight (N = 4-6 per group). C. The proportion of CD45 + cells in the IHL as determined by flow cytometry (N = 4-6 per group). D. (Left panel) CD4 + T cells abundance in gated CD45 + immune cells, and (Right panel ) the proportion of LIGHT + cells in the CD4 + T cell fraction (N = 3 per group). E. (Left panel) NK T cell abundance in gated CD45 + immune cells, and (Right panel ) proportion of LIGHT + cells in the NK T cell fraction (N = 4-6 per group). F. LIGHT positivity in CD4 + T cell subsets, Th1, Th2, Th17, and T-reg, was determined by flow cytometry (N = 3 per group). G. WT primary mouse splenocytes when stimulated with PMA/ionomycin or vehicle display (left panel) a similar proportion of CD3 + CD4 + T cells, (middle panel) an increased abundance of IFNγ + CD 4 + T cells and (right panel) an increased proportion of LIGHT + CD4 + T cells (N = 3 per group). H. The expression of pro-fibrogenic genes ACTA2, COL1A1, PDGFRA and CTGF is elevated in primary human hepatic stellate cells when stimulated with recombinant LIGHT (N = 3 per group). * - p < 0.05, ** - p < 0.01, *** - p < 0.005, **** - p < 0.001.

Article Snippet: Cells were rested for 4 hours and stimulated with medium containing vehicle or recombinant human LIGHT (664-LI, R&D systems, Minneapolis, MN) for 72 hours refreshing the medium at 48 hours.

Techniques: Isolation, Control, Flow Cytometry, Expressing, Recombinant

Journal: Oncology Reports

Article Title: Loperamide reverses 5-FU resistance in colorectal cancer by activating autophagy

doi: 10.3892/or.2025.8966

Figure Lengend Snippet: LOP enhances the inhibitory effects of 5-FU on HCT8R cells through autophagy activation. (A) Western blot analysis of changes in LC3 and Beclin protein expression in HCT8R cells treated with LOP and 5-FU. (B) Confocal microscopy images, showing the generation of autophagic vesicles in HCT8R cells transfected with the EGFP-LC3 plasmid, and treated with LOP and 5-FU. (C) Western blot analysis of Beclin protein expression in HCT8R cells treated with or without 3-MA. (D) Statistical analysis of changes in Beclin expression in HCT8R cells treated with or without 3-MA. (E) Transwell assays were performed, assessing the migratory capability of HCT8R cells treated with LOP, 5-FU, with or without 3-MA. *P<0.05, **P<0.01 (n=3). 3-MA, 3-methyladenine; 5-FU, 5-fluorouracil; DMSO, dimethyl sulfoxide; LC3, microtubule-associated protein 1 light chain 3; LOP, loperamide; ns, not significant.

Article Snippet: Transfection was subsequently performed using Lipo8000 TM reagent (Beyotime Institute of Biotechnology) in combination with 2 μg pEGFP (cat. no. 165830; Addgene, Inc.) or pEGFP-microtubule-associated protein 1 light chain 3 (LC3) plasmid (cat. no. 24920; Addgene, Inc.) and Opti-MEM TM serum-free medium (Gibco; Thermo Fisher Scientific Inc.).

Techniques: Activation Assay, Western Blot, Expressing, Confocal Microscopy, Transfection, Plasmid Preparation